All publications
Establishment of a continuous inline-monitored nano-production line using the Microfluidizer® technology for the fabrication of safe lipid-based nanoparticles
Macedonian Pharmaceutical Bulletin - Sep 2023
Nano-drug delivery systems (NDDS) serve as a promising platform in modern medicine, facilitating the targeted delivery of drugs due to their versatile size, structure, and composition. However, the current industrial production of NDDS relies on cumbersome batch processes that are costly, difficult to scale, and lack adequate control. This study focuses on enhancing industrial-scale production through the development and testing of a continuous top-down production line, utilizing scalable Microfluidizer® technology for solvent-free preparation of lipid-based nanoparticles.
Lipid-based nanocarriers for oral delivery of peptides
OCL, Volume 29, 1 https://doi.org/10.1051/ocl/2021040 - Jan 2022
Solid lipid Nanocarriers diffuse effectively through mucus and enter intestinal cells – but where is my peptide?
International Journal of Pharmaceutics, Volume 586, 119581 https://doi.org/10.1016/j.ijpharm.2020.119581 - Aug 2020
This study aimed to evaluate the potential of Hydrophobic Ion Pairing combined with Solid Lipid Nanoparticles (SLN) and Nanostructured Lipid Carriers (NLC) to improve peptide transport across the intestinal border using Caco-2 cell monolayers (enterocyte-like model) and Caco-2/HT29-MTX co-cultured monolayers (mucin-secreting model).
This study showed that hydrophobic ion pairing enabled high encapsulation efficiency of peptide in SLN and NLC. The lipid-based nanocarriers were highly internalized by Caco-2 cell monolayers and were able to cross the mucus barrier (Caco-2/HT29-MTX cell model). However, the peptide intestinal transport was limited by an extensive release from the carriers.
A proof-of-concept for developing oral lipidized peptide Nanostructured Lipid Carrier formulations
Journal of Drug Delivery Science and Technology, Volume 54, 101394 https://doi.org/10.1016/j.jddst.2019.101394 - Dec 2019
A proof-of-concept for developing oral lipidized peptide Nanostructured Lipid Carrier formulations
In-vitro evaluation of solid lipid nanoparticles: Ability to encapsulate, release and ensure effective protection of peptides in the gastrointestinal tract
International Journal of Pharmaceutics, Volume 565, Pages 409-418 - Jun 2019
Lipid-based nanosuspensions for oral delivery of peptides, a critical review
International Journal of Pharmaceutics, Volume 541, Issues 1–2, Pages 117–135 - Apr 2018
In this review article, the authors describe:
the different techniques used to prepare Solid Lipid Nanoparticles and Nanostructured Lipid Carriers
the methods used to assess the protective effects of the carriers on peptides
the techniques used to measure the permeability enhancement
Evaluation of the digestibility of solid lipid nanoparticles of glyceryl dibehenate produced by two techniques: Ultrasonication and spray-flash evaporation
European Journal of Pharmaceutical Sciences , Volume 111, Pages 91-95 https://doi.org/10.1016/j.ejps.2017.09.049 - Jan 2018
In this study, solid lipid nanoparticles (SLN) of glyceryl dibehenate are produced by two methods and evaluated for digestion using the in vitro lipolysis test.
By ultrasonication, SLN have a mean particle size of 180 nm and showed a limited digestion by lipase.
By spray-flash evaporation, SLN particle size ranges from 235 to 411 nm, depending on process conditions and were not digested by lipase.