Publications on solid lipid nanoparticles
Lipid-based nanocarriers for oral delivery of peptides
Camille Dumont
Solid lipid nanocarriers to increase oral bioavailability of the peptide Leuprolide
Camille Dumont, Vincent Jannin, Sandrine Bourgeois, Ana Beloqui, Véronique Préat, Hatem Fessi
Poster PBP 2021
Solid lipid Nanocarriers diffuse effectively through mucus and enter intestinal cells – but where is my peptide?
Camille Dumont, Ana Beloqui, Cédric Miolane, Sandrine Bourgeois, Véronique Préat, Hatem Fessi, Vincent Jannin
International Journal of Pharmaceutics, Volume 586, 30 August 2020
This study aimed to evaluate the potential of Hydrophobic Ion Pairing combined with Solid Lipid Nanoparticles (SLN) and Nanostructured Lipid Carriers (NLC) to improve peptide transport across the intestinal border using Caco-2 cell monolayers (enterocyte-like model) and Caco-2/HT29-MTX co-cultured monolayers (mucin-secreting model).
This study showed that hydrophobic ion pairing enabled high encapsulation efficiency of peptide in SLN and NLC. The lipid-based nanocarriers were highly internalized by Caco-2 cell monolayers and were able to cross the mucus barrier (Caco-2/HT29-MTX cell model). However, the peptide intestinal transport was limited by an extensive release from the carriers.
Solid lipid nanocarrier development toolbox for increasing oral bioavailability of API
Dr Camille Dumont
American Pharma Review, May-June 2020 issue, pp 69-75
In this comprehensive article, the author describes the latest tools available to develop solid lipid nanoparticles: how to prepare and characterize solid lipid nanoparticles, how to evaluate encapsulation efficiency and intestinal permebility.
A proof-of-concept for developing oral lipidized peptide Nanostructured Lipid Carrier formulations
Camille Dumont, Vincent Jannin, Cédric Miolane, Quentin Lelong, Jean-Pierre Valour, Sebastien Urbaniak, Hatem Fessib, Sandrine Bourgeois
Journal of Drug Delivery Science and Technology (article in press)
In this study, the authors developed a protocol to produce reproducible solid lipid nanoparticles by high pressure homogeneization. The possibility to encapsulate peptides was also investigated.
In Vitro Studies Indicate Promising Properties Of Solid Lipid Nanosuspensions for oral peptide delivery
Camille Dumont, Ana Beloqui, Sandrine Bourgeois, Véronique Préat, Hatem Fessi, Vincent Jannin
Poster CRS 2019
In-vitro evaluation of solid lipid nanoparticles: Ability to encapsulate, release and ensure effective protection of peptides in the gastrointestinal tract
Camille Dumont, Sandrine Bourgeois, Hatem Fessib, Pierre-Yves Dugas, Vincent Jannin
International Journal of Pharmaceutics, Volume 565, 30 June 2019, Pages 409-418
Lipid-based nanosuspensions for oral delivery of peptides, a critical review
Camille Dumont, Sandrine Bourgeois, Hatem Fessi, Vincent Jannin
- the different techniques used to prepare Solid Lipid Nanoparticles and Nanostructured Lipid Carriers
- the methods used to assess the protective effects of the carriers on peptides
- the techniques used to measure the permeability enhancement
In vitro evaluation of leuprolide-containing solid lipid-based nanosuspensions: ability to encapsulate, release, protect and permeate in the gastrointestinal tract
Dumont C., Jannin V., Beloqui A., Préat V., Fessi H.c, Bourgeois S.
In this study the authors obtained solid lipid nanoparticles using high pressure homogeneization, in which leuprolide was included as an ion pair. The nanoparticles were shown to be internalized by Caco-2 and Caco-2/HT29-MTX cell monolayers.
Evaluation of the digestibility of solid lipid nanoparticles of glyceryl dibehenate produced by two techniques: Ultrasonication and spray-flash evaporation
Published online October 2017
European Journal of Pharmaceutical Sciences , Volume 111, 1 January 2018, Pages 91-95
In this study, solid lipid nanoparticles (SLN) of glyceryl dibehenate are produced by two methods and evaluated for digestion using the in vitro lipolysis test.
- By ultrasonication, SLN have a mean particle size of 180 nm and showed a limited digestion by lipase.
- By spray-flash evaporation, SLN particle size ranges from 235 to 411 nm, depending on process conditions and were not digested by lipase.
